Factors confirmed to be significant for discriminating tumor stage (I/II vs. III/IV) by multivariate analysis were tumor markers (CEA and CA19-9), tumor diameter ≥ 60 mm, macroscopic type (type 0, type 2, type 3, and type 4), mucinous histology, and infiltration of the esophagus. The gene discussed is CEACAM5; the disease is neoplasm.