In addition, we detected NHE1 expression in sensitive cells and chemoresistant cell lines, including breast cancer(MCF-7 and MCF-7/ADR) and ovarian cancer(A2780 and A2780/PTX) by western blot analysis(Fig. 1e) and RT-PCR(Fig. 1f). The gene discussed is SLC9A1; the disease is ovarian carcinoma.