In all examined melanoma cell lines, inhibitors:: AKT – MK-2206 (2 μM), MEK – AS-703026 (10 μM), PI3K – LY294002 (20 μM), ERK1/2 – U0126 (10 μM), mTOR – everolimus (20 nM, 2 μM, 5 μM and 10 μM) showed no cytotoxicity effect in the 24 h of treatment. Here, MTOR is linked to melanoma.