We previously observed that Rab25 expression was higher in NSCLC tissue than in adjacent normal tissue, and high Rab25 expression was significantly correlated to worse prognosis.11 To further explore the role of Rab25 in EGFR‐TKI resistance, we examined the expression of Rab25 and EGFR signalling in the EGFR‐TKI‐sensitive PC9 and HCC827 cells and the resistant PC9/ER and HCC827/ER subclones. The gene discussed is RAB25; the disease is non-small cell lung carcinoma.