The near infrared imaging and tomography results suggested that the selected Fc-fusion-NIR tracer performed well in distinguishing CD248 expressing tumor grafts from normal organs and control grafts in vivo [113].CD248 expression was analyzed in clinical sarcoma specimens processed by standard formalin-fixed paraffin embedded techniques collected including 19 human sarcoma subtypes and 8 human sarcoma cell lines. The gene discussed is CD248; the disease is sarcoma.