Nevertheless, despite the depletion of the majority of the B cell compartment, anti-CD20 mAb treatment did not increase the activation or function of splenic or intracerebral CD8+ T cells during 4th infection (Figures 5D,E), with granzyme B expression by CD8+ T cells in anti-CD20mAb and control Ab treated 4X infected mice being significantly lower than in 1X infection (Figure S4). This evidence concerns the gene CD8A and infection.