However, in Unc93b1 mutant, and Tlr13−/− BMDMs production of IL-6 and TNFα upon infection with S. pyogenes ATCC12344 and M49 was almost abrogated at lower MOIs (Figures 1A,B and Supplementary Figure 2), indicating a non-redundant role of RNA/TLR13 recognition at low bacterial burden. This evidence concerns the gene IL6 and infection.