Infection of neutrophils isolated from WT, Unc93b1 3d, and Tlr13−/− mice with S. pyogenes ATCC12344 revealed a similar stimulation pattern as observed in BMDCs: While in neutrophils lacking functional UNC93b1, TNFα production triggered by S. pyogenes was strongly impaired and IL-12p40 secretion was even completely abolished, the secretion of both cytokines was less attenuated in Tlr13−/− cells, especially in case of IL-12p40 (Figures 3D,E). The gene discussed is TNF; the disease is infection.