ZEB1 and neoplasm: MTT and cell invasion assays showed that knockdown of ZEB1 by the introduction of ZEB1 siRNA suppressed MG63 and HOS cell proliferation (Figure 5B, P < 0.05) and invasion (Figure 5C, P < 0.05), suggesting that negative regulation of ZEB1 may mediate the tumor suppressive effects of miR-409-3p in OS cells.