MYC and neoplasm: Therefore, we next sought to determine if combining MYC suppression with JQ1 treatment would lead to additive anti-tumor activity in cells in which JQ1 failed to suppress MYC. To determine that, we used RNAi to deplete MYC in PC3 and DU145 cells and then treated the cells +/−500 nM JQ1 for 96 hours (Fig. 4).