MYOD1 and Duchenne muscular dystrophy: On the 14th day after differentiation, we could clearly demonstrate exon-skipping in a dose-dependent manner at the mRNA and protein level in DZNep-treated MYOD1-UDCs and MYOD1-Fibs from both DMD patients by RT-PCR and immunoblotting, respectively (Fig. 4A–D, Supplementary Fig. S6A–D).