Finally, whereas the number of putative U251 CD133+ colon cancer stem cells (4%) was unaffected (Fig. 3e), the number of clonal spheroids was drastically reduced upon NANEP5 expression either upon diluted single cell or in bulk conditions (Fig. 3f), much as reported for NANOG kd18,27. This evidence concerns the gene PROM1 and malignant colon neoplasm.