Moreover, obese pre-DM under metformin therapy showed higher values of SIRT6 expression (p < 0.05) and lower values of NF-κB, PPAR-γ, and SREBP-1 protein levels (p < 0.05) compared to obese pre-DM not-metformin users (Figure 1). This evidence concerns the gene SIRT6 and diabetes mellitus.