In contrast to this, another study found that up to 10 μM, THC had no effect on the growth and viability of the CB1/CB2 positive HCmel12 (established from a primary, 7,12-dimethylbenz(a)anthracene-induced melanoma of HGF-CDK4R24C mouse [408]) and B16 melanoma cells in vitro as determined by trypan blue exclusion assay [392]. The gene discussed is HGF; the disease is melanoma.