When assessing the effects of the eCBs, AEA was found to by cytotoxic (IC50: 5.87 ± 0.7 μM) in human A375 melanoma cells in a CB1-, COX2-, and a caspase-dependent manner, but neither CB2, nor TRPV1 antagonists influenced the effect. The gene discussed is TRPV1; the disease is melanoma.