We selected four DEmRNAs (Wbscr27, Sfrp5, Adig, and Saa3), DEcircRNAs- chr7:67264864–67268400:- and DElncRNA-ENSMUST00000169194, which are most relevant to obesity, for use in verifying RNA-seq results using qRT-PCR. The gene discussed is SAA3P; the disease is obesity due to melanocortin 4 receptor deficiency.