Consistent with the morphological analysis, gene set enrichment analysis (GSEA) revealed that the genes downregulated in CD34− HSC following Regnase-1 deletion were statistically significantly similar to an a priori-defined set of genes in HSCs from Pu.1-deficient mice, taken to model AML (Fig. 5g). This evidence concerns the gene ZC3H12A and acute myeloid leukemia.