Human NSCLC cell lines including H1975 (EGFR L858R/T790M) and HCC827 (EGFR Del 19) were obtained from State Key Laboratory of Biotherapy, Sichuan University, and maintained with RPMI 1640 medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (SIGMA, USA) under a moist atmosphere of 5% CO2 at 37 °C. This evidence concerns the gene EGFR and non-small cell lung carcinoma.