Since we could only show a robust Wnt3a expression pattern in cell lines, derived from normal squamous epithelium, EPC-1 and EPC-2 (Fig. 1a), as well as a weaker WNT3A staining in metaplastic, HGIN and esophageal adenocarcinoma specimens (Fig. 7), we were able to demonstrate a silencing of Wnt3a with the progression of the Barrett’s esophagus in vitro (cell lines) and in vivo (immunohistochemistry). This evidence concerns the gene WNT3A and esophageal adenocarcinoma.