It is not surprising to know that treatment with nintedanib, which targets many growth factor receptors, was associated with downregulation of E2F1. Our interaction analysis suggests that upregulation of hsa-miR-486-3p might have an interaction with downregulation of E2F1 in nintedanib-treated IPF fibroblasts. The gene discussed is E2F1; the disease is idiopathic pulmonary fibrosis.