To determine whether circulating TNF blockage can attenuate subcutaneous GAS infection-induced central nervous system inflammation, we treated infected mice with three doses (30 min before, 3 h after infection, and 6 h after infection) of a dominant-negative inhibitor of TNF (DN-TNF) and detected the luciferase signal at 3, 24, and 48 hpi. This evidence concerns the gene TNF and infection.