To evaluate the effects of the Th2 cytokines, IL-4 and IL-13, on TMEM16A expression in the mature bronchial epithelium, we cultured normal and COPD human bronchial epithelial (HBE) cells at an air-liquid interface (ALI) to generate a polarized, pseudostratified bronchial epithelium composed of basal cells, secretory cells, and ciliated cells. This evidence concerns the gene ANO1 and chronic obstructive pulmonary disease.