The p53 protein cooperates with PTEN and could be an essential blockage in the development of mammary tumours6; thus, we can comprehend that the therapeutic efficiency of anticancer agents strongly depends on their detailed mechanisms to trigger apoptosis in targeted cancer cells.24 As p53 plays a pivotal role in regulating tumour apoptosis, our study elucidated the prominence of the p53‐dependent mechanism on the anti‐HCC effect of GRA16. The gene discussed is TP53; the disease is hepatocellular carcinoma.