Among the 77 patients with ESCC, the patient characteristics of gender, age, age group, smoking history, drinking history, family history of ESCC, lesion number, T stage, N stage, differentiation, TNM stage, perineural invasion, LN positivity, and growth of new vessels were compared with the germline mutation status, which comprised homologous recombination deficiency (HRD) VUS status, MMR VUS status, SLX4 rare VUS status, TSC2 rare VUS status, CYP21A2 pathogenic mutation status, VUS_group, and P_group (Figure 4). The gene discussed is TSC2; the disease is esophageal squamous cell carcinoma.