Western blot analysis of tumor lysates demonstrated similar results as those observed by IHC staining, with ACVR1 R206H, H3.1K27M; PDGF-A; p53 deficient mutant tumors having significantly higher protein levels of Id1, Hes1, and CD31 than other tumor types, with ACVR1 R206H contributing most to these higher levels (Fig. 5b–d). Here, TP53 is linked to neoplasm.