To study the role of ACVR1 in DIPG pathogenesis, brainstem progenitors were infected with three common ACVR1 mutants: R206H, G328V, and G328E which resulted in differential effects on proliferation and cell survival with ACVR1 R206H being the most potent mutation, ACVR1 G328V being intermediate, and ACVR1 G328E being the least potent. This evidence concerns the gene ACVR1 and diffuse intrinsic pontine glioma.