To further characterize the glioma-like lesions, all groups except ACVR1 G328V, H3.1K27M, and Cre (because they were too small) were stained for Nestin, Olig2, and GFAP as these markers have been expressed in at least a subset of human DIPGs20 (Fig. 3). This evidence concerns the gene NES and central nervous system cancer.