CD86 and neoplasm: As expected, the tumor-bearing DCs co-cultured with cryo-thermal macrophages were highly matured, as demonstrated by the increased percentage of CD11c+CD86+MHC II+ DCs along with the higher relative expression of IL-6, TNF-α, CXCL10, IL-1β, IL-12p40, and IL-15 mRNA (Supplementary Fig. 6A-B).