IL2RA and Autoimmunity: To determine IL-2Rα-dependent gene programs operative in thymic and peripheral Tregs, RNA-seq was performed on FACS-purified, CD25-deficient Tregs and reporter controls from the thymus of “cured” CD25cKO mice and the spleen of tamoxifen-induced CD25flox/Foxp3eGFP-Cre-ERT2/R26Y mice, to eliminate any confounding effects of autoimmunity (Fig. 6a).