We also found that in spontaneous tumor tissue, ARG1 positive TAMs were enriched in hypoxic regions (GLUT1 positive regions) and accompanied with reduced mitochondrial marker TIMM23 (Fig. 7h), further indicating that hypoxia-induced reduction of NEMG expression via NRF1 degradation contributes to the polarization of TAMs. This evidence concerns the gene NRF1 and neoplasm.