NRF1 and neoplasm: Western blot analysis of the xenograft tumor tissues showed that the ratio of activated MLKL (p-MLKL Ser358) to total MLKL was decreased in reconstituted wild-type NRF1 or K230R cells comparing with control cells (Supplementary Fig. 8), indicating that the necrosis in the tissues is not due to MLKL-mediated necroptosis.