G3BP1 and neuroblastoma: Therefore, we used CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) gene editing technique to insert a GFP tag in frame to the N terminus of the G3BP1 gene in the human neuroblastoma cell line SH-SY5Y (Fig. 2a, Supplementary Fig. 2a, b).