In the second set of experiment, we transplanted sh-NPTX2 or NPTX2-HA CRC cells into the cecum of nude mice to establish an orthotopic tumor model and found that the tumor weight significantly decreased after NPTX2 knockdown but obviously increased after NPTX2 overexpression (Fig. 3e). This evidence concerns the gene NPTX2 and colorectal carcinoma.