To confirm the correlation between NPTX2 and Wnt/β-catenin pathways, we conducted a TOP/FOP luciferase activity assay and found that the knockdown of NPTX2 expression obviously inhibited the Wnt/β-catenin pathway, while NPTX2 overexpression activated the Wnt/β-catenin pathway in CRC (Fig. 4b and Fig. S4A). The gene discussed is NPTX2; the disease is colorectal carcinoma.