To further substantiate the shRNA-mediated CgA knockdown effect in neuroblastoma proliferation, we performed rescue experiment using an shRNA-resistant CgA plasmid which contained optimized CgA codon sequences to avoid recognition and degradation by the CgA shRNA [CgA mRNA expression (fold change), vector versus CgA Rescue, 1.0±0.1 versus 11.3±0.04, P<0.01, Fig. 1G, left panel]. Here, CGA is linked to neuroblastoma.