CGA and neuroblastoma: We observed that knockdown of CgA in neuroblastoma SH-SY5Y cells resulted in a reduction in both IGF-II and IGFBP-2 mRNA expression (relative mRNA expression, nonsense versus shRNA CgA, IGF-II 0.9±0.1 versus 0.1±0.01, P<0.01, Fig. 5A; IGFBP-2 0.9±0.2 versus 0.1±0.1, P<0.01, Fig. 5B).