To elucidate the pathological effects of FAT4 in CRC cells, we examined the proliferation of these cells through MTT assays and found that the proliferation capacity of SW480 cells with suppressed FAT4 expression was higher than that of control SW480 cells; however, the overexpression of FAT4 in HCT116 and LOVO cells led to a decline in the proliferation ability of these cells (Fig. 1e). The gene discussed is FAT4; the disease is colorectal carcinoma.