In this study, we compared the molecular and cellular functions of FAT4 between autophagy and the EMT and observed that Twist1 is downregulated in CRC cells treated with the mTOR inhibitor Troin1 and that Twist1 expression is upregulated by treatment with the mTOR promoter MHY1485, which can decrease the level of E-cadherin. The gene discussed is FAT4; the disease is colorectal carcinoma.