Sample sizes of the initial dataset, and for samples that were able to be included at each stage (mutation burden calling, neoepitope load estimation, and then gene expression analysis), are reported for each of the three breast cancer subtype categories (ER/PR(+)HER-2(−), HER-2(+), TNBC) (PDF 56 kb). The gene discussed is ESR1; the disease is breast carcinoma.