The response to megapool stimulation, induced by M. tb infection, was characterized by a predominance of IFNγ+TNF+IL-2+ polyfunctional CD4 T cells, whereas the BCG-induced response comprised low proportions of polyfunctional CD4 T cells; responses induced by the novel TB vaccines fell in between the megapool and BCG responses. The gene discussed is TNF; the disease is tuberculosis.