To further investigate the clinical relevance of LKB1 loss with osteogenic sarcoma and the therapeutic effects of rapamycin treatment in human osteosarcoma, we used LKB1 shRNA (shLKB1) lentivirus to knock down LKB1 expression in the human osteosarcoma cell line HOS-MNNG cell, which has been reported to express high levels of LKB1 (24, 41). Here, STK11 is linked to osteosarcoma.