To characterize the effect of human plasma on sporozoite gliding motility that is independent of naturally acquired immunity, sporozoites were incubated in the presence of plasma from malaria-naive individuals (n=5) and showed an average gliding trail surface of 1,427(95% CI 646.6-2,207) pixels, significantly greater than the gliding trail surface of sporozoites incubated with 30 μg/ml of a monoclonal anti-CSP antibody, our positive control, 141 (95% CI 71.9-225.4) pixels. This evidence concerns the gene DNAJC5 and malaria.