Trizol法提取NSCLC细胞A549/DDP的总RNA,利用RevertAid First逆转录试剂盒得到cDNA,以cDNA为模板,分别以MRTF-A、GAPDH及HOTAIR引物进行qRT-PCR反应。MRTF-A上游引物:5’-ACCGTGACCAATAAGAATGC-3’,下游引物:5’-CCGCTCTGAATGAGAATGTC-3’。HOTAIR上游引物:5’-TAGGCAAATGTCAGAGGGTT-3’,下游引物:5’-ACACAAGTAGCAGGGAAAGG-3’。GAPDH引物,上游引物:5’-CGAG ATCCCTCCAAAATCAA-3’,下游引物:5’-TTCACACCCATG AC GAACAT-3’。. The gene discussed is GAPDH; the disease is non-small cell lung carcinoma.