BEST1 and melanoma: To validate RPE-specific expression of fluorescence markers from the new AAV vectors, melanoma cells, which enable transcription from VMD2-driven expression cassettes in vitro,34 were transfected with p/miR(Irr)-AsR-PE, p/miR(5,B,7)-AsR-PE, and p/miR(5,B,7)-PEDF-PE (Figure 1B).