In this study, we found that knocking down lncRNA‐ZEB2‐AS1 results in the down‐regulation of ZEB2 expression through the PI3K/Akt/GSK3β/Zeb2 signaling pathway, inactivation of AKT and GSK3β phosphorylation, inhibition of EMT in breast cancer. The gene discussed is ZEB2; the disease is breast cancer.