On a complementary approach, by using a pCMV6-TLR4 construct we overexpressed TLR4 in H929 (Fig. 3c) and U266 (Fig. 3d1) cells, as these MM cell lines express medium or low (compared to JJN3 cells) TLR4 levels; notably, in spite of very high TLR4 mRNA expression levels in transfected cells (Fig. 3c,d1) the TLR4 protein was only mildly overexpressed (Fig. 3d2, Suppl. The gene discussed is TLR4; the disease is Miyoshi myopathy.