DDIT3 and Miyoshi myopathy: Next, in order to determine whether LPS could suppress CHOP expression even under conditions of sustained ER stress, U266 and JJN3 cells were treated with 1 μg/ml LPS for 2 h and were then subjected to ER stress conditions by treatment with Tunicamycin (TM) for 24 h; these conditions are known to upregulate CHOP in MM cells8,20.