Proof-of-concept of 18F-labeling with heat/solvent-sensitive biomolecules in one step is demonstrated by biomolecular tracers include 18F-labeled human serum albumin (HSA) for blood pool imaging and 18F-labeled c(RGDyk) which targets the integrin αvβ3 receptor of the U87MG cell (glioblastoma). This evidence concerns the gene ALB and glioblastoma.