AICDA and colon adenocarcinoma: Using CRISPR/Cas9-based genome editing, we introduced a cassette encoding mini-AID (mAID) and fluorescent protein mClover (mAID+mClover) at the initiation site of the endogenous RNAPII gene locus (POLR2A; Fig. 5 B) in human colon adenocarcinoma DLD-1 cells expressing OsTIR1, which is involved in the induced degradation process (Fig. 5 A; Natsume et al., 2016; Yesbolatova et al., 2019Preprint).