We then detected the mRNA level of ZEB1-AS1 (Fig. 3g) in BLCA cell lines and found that ZEB1-AS1 expression was high in some high grade BLCA cell lines (Tccsup, T24, and UM-UC3), in which the expression of miR-200b was low, while the expression of FSCN1 was high. This evidence concerns the gene ZEB1 and bladder transitional cell carcinoma.