We then detected the mRNA levels of FSCN1 and miR-200b in BLCA cell lines and found that FSCN1 was highly expressed in some high grade BLCA cell lines (Tccsup, T24, UM-UC3), in which the expression level of miR-200b was low (Fig. 1f and g). This evidence concerns the gene FSCN1 and bladder transitional cell carcinoma.