The ability of the PRC2 complex to bind to the HOXD3 promoter was significantly increased in the CRC cells overexpressing HOXD-AS1 compared with the control cells expressing endogenous levels of HOXD-AS1 (Fig. 3h and Additional file 6: Figure S4b), suggesting that anti-SUZ12- and anti-EZH2-antibodies can both bind to the HOXD3 promoter region. This evidence concerns the gene HAGLR and colorectal carcinoma.