To determine the effects of altered cohesin expression in vivo, we introduced both SMC1A wild-type gene and SMC1A c.A2027G (leading to p.E676G change, located in the coiled-coil domain near the hinge domain) mutation, which induced chromosomal instability when transiently transfected in vitro [30], in HCT116 cells, a near-diploid human colorectal cancer cell line with stable karyotype. This evidence concerns the gene SMC1A and colorectal cancer.