NRF1 and status epilepticus: To demonstrate the temporal change of mitochondrial biogenesis machinery expression following experimental status epilepticus, we first showed nuclear respiratory factor 1 (NRF1) expression in total protein prepared from the right hippocampal CA3 subfield, which revealed a significant increase of expression of NRF1 from 3 to 24 h, with peak level at 6 h after KA treatment (Figure 2A).