Rb1 attenuates myocardial ischemia/reperfusion injury and reduces infarct size, cardiomyocyte apoptosis, and caspase-3 activity, partly by inhibiting oxidative stress and apoptosis, enhancing eNOS expression, and increasing NO concentrations, as well as activating the PI3K/Akt pathway. This evidence concerns the gene RB1 and myocardial ischemia.