Next, we determined whether NR4A1 alone had some effects on TRAIL‐induced apoptosis in HCC cells, because a recent study reported that TNF‐α rapidly induces NR4A1 expression in breast cancer cells.19 To test whether TRAIL has a similar ability to induce NR4A1 expression in hepatoma cells, we treated LH86 and Huh7 cells with TRAIL and examined NR4A1 expression by Western blot. The gene discussed is TNFSF10; the disease is hepatocellular carcinoma.