The aim of the present study was to assess the expression of all the proteins from SLIT–ROBO family (SLIT1, SLIT2, SLIT3, ROBO1, ROBO2, ROBO3, and ROBO4) in the BM biopsy of AML patients by immunohistochemical staining. This evidence concerns the gene ROBO3 and acute myeloid leukemia.