Bearing in mind the interaction between AFAP1-AS1 and miR-384, our results obtained further suggested that AFAP1-AS1 could act to regulate the expression of ACVR1 by competitively binding to miR-384, which signifies the role of AFAP1-AS1 in the regulatory network involving PC tumorigenesis. This evidence concerns the gene ACVR1 and pachyonychia congenita.