Additionally, the mRNA and protein expression of the CSC markers (Oct4, ABCG2, Nestin, CK19 and CD133) were decreased in the PC cells when AFAP1-AS1 was knocked-down, which ultimately demonstrated that the stemness of the PC cells was attenuated when AFAP1-AS1 was reduced. This evidence concerns the gene PROM1 and pachyonychia congenita.