Interleukin 10 deficient mice, bacterial translocation and overgrowth, and mesenteric lymph node became the research foci since 1998 and then the intestinal epithelial cell; 16s rRNA sequencing became a research focus since 2004, followed by toll like receptor and innate immunity, marking microbiome research entering the new era; regarding the bursts with most recent onset: bile acid, obesity, and Akkermansia muciniphila were the strongest bursts that started in 2016. The gene discussed is IL10; the disease is obesity due to melanocortin 4 receptor deficiency.