Our results show that longer exposure to a standard HFD containing 0.2% cholesterol also resulted in NAFLD/NASH, since ApoE-/- mice fed an HFD for 12 weeks had significant lipid deposition in the liver, which was accompanied by fibrotic changes and the expression of the macrophage marker F4/80. Here, APOE is linked to metabolic dysfunction-associated steatohepatitis.